Metabolic response induced by endophytic fungi and bacteria in H. marrubioides Epling in vitro microplants

Química Nova

Januário, Ana Helena¹  Soares, Marcos Antônio¹  Silva, Fabiano Guimarães¹  Lima, William Cardoso¹  Universidade de Franca, Franca, Brasil¹  Cunha, Wilson Roberto¹  Universidade Federal de Mato Grosso, Cuiabá, Brasil¹  Pedroso, Rita Cássia Nascimento¹  Dias, Herbert Júnior¹  Instituto Federal de Educação, Ciência e Tecnologia Goiano, Rio Verde, Brasil¹  Vitorino, Luciana Cristina¹  Pauletti, Patrícia Mendonça¹  Crotti, Antônio Eduardo Miller¹  Silva, Marolí Rodrigues¹  Silva, Márcio Luís Andrade e¹ 

关键词: in vitro culture;    Hyptis marrubioides;    Lamiaceae.;   

DOI  :  10.1590/S0100-40422013000700016

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Hyptis marrubioides Epling is a native plant from Brazilian Cerrado. In this paper, the response of in vitro microplants of this species to inoculation with bacterial and fungal endophytic isolates is evaluated. HPLC-DAD analysis showed the presence of 3,4-O-(Z)-dicaffeoylquinic acid and quercetin-7-O-glucoside as the main components. GC/MS analysis demonstrated that the sesquiterpenes τ-cadinol and caryophyllene oxide were only produced in microplants inoculated with endophytic bacteria, while methyl hexadecanoate, methyl heptadecanoate and methyl (Z,Z,Z) 9,12,15-octadecatrienoate and the triterpene methyl 3β-hydroxy-urs-12-en-28-oate were overexpressed only when the microplant was treated with endophytic fungi.

【 授权许可】

Unknown