Metabolic response induced by endophytic fungi and bacteria in H. marrubioides Epling in vitro microplants

Quimica nova

Vitorino, Luciana Cristina¹  Silva, Fabiano Guimarães¹  Soares, Marcos Antônio²  Lima, William Cardoso²  Pedroso, Rita Cássia Nascimento³  Dias, Herbert Júnior³  Januário, Ana Helena³  Pauletti, Patrícia Mendonça³  Crotti, Antônio Eduardo Miller³  Silva, Marolí Rodrigues³  Silva, Márcio Luís Andrade e³  Cunha, Wilson Roberto³ 

[1] Instituto Federal de Educação, Ciência e Tecnologia Goiano, Rio Verde, Brasil;Universidade Federal de Mato Grosso, Cuiabá, Brasil;Universidade de Franca, Franca, Brasil

关键词: in vitro culture;    Hyptis marrubioides;    Lamiaceae.;   

DOI  :  10.1590/S0100-40422013000700016

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Hyptis marrubioidesEpling is a native plant from Brazilian Cerrado. In this paper, the responseof in vitro microplants of this species to inoculation with bacterialand fungal endophytic isolates is evaluated. HPLC-DAD analysis showed the presenceof 3,4-O-(Z)-dicaffeoylquinic acid and quercetin-7-O-glucosideas the main components. GC/MS analysis demonstrated that the sesquiterpenesτ-cadinoland caryophyllene oxide were only produced in microplants inoculated with endophyticbacteria, while methyl hexadecanoate, methyl heptadecanoate and methyl (Z,Z,Z)9,12,15-octadecatrienoate and the triterpene methyl 3β-hydroxy-urs-12-en-28-oatewere overexpressed only when the microplant was treated with endophytic fungi.

【 授权许可】

CC BY