Investigação eletroquímica e calorimétrica da interação de novos agentes antitumorais biscatiônicos com DNA

Química Nova

Láuris Lucia Da Silva¹  Claudio Luis Donnici¹  Júlio César Dias Lopes¹  Marília Oliveira Fonseca Goulart²  Fabiane Caxico De Abreu²  Francine Santos De Paula²  Carlos E. Salas Bravo²  Marcelo Matos Santoro²  Ângelo Márcio Leite Denadai²  Alexandre Martins Costa Santos²  Carlos Alberto Montanari² 

[1] ,Universidade Federal de Minas Gerais Instituto de Ciências Exatas Departamento de QuímicaBelo Horizonte MG ,Brasil

关键词: biscationic compounds and interaction with DNA;    isothermal titration calorimetry;    electrochemical biosensor;   

DOI  :  10.1590/S0100-40422012000700007

来源: SciELO

【 摘 要 】

Biscationic amidines bind in the DNA minor groove and present biological activity against a range of infectious diseases. Two new biscationic compounds (bis-α,ω-S-thioureido, amino and sulfide analogues) were synthesized in good yields and fully characterized, and their interaction with DNA was also investigated. Isothermal titration calorimetry (ITC) was used to measure the thermodynamic properties of binding interactions between DNA and these ligands. A double stranded calf thymus DNA immobilized on an electrode surface was used to study the possible DNA-interacting abilities of these compounds towards dsDNA in situ. A remarkable interaction of these compounds with DNA was demonstrated and their potential application as anticancer agents was furthered.

【 授权许可】

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