Electrochemical and calorimetric investigation of interaction of novel biscationic anticancer agents with DNA

Quimica nova

Denadai, Ângelo Márcio Leite¹  Paula, Francine Santos de²  Abreu, Fabiane Caxico de²  Goulart, Marília Oliveira Fonseca²  Donnici, Claudio Luis³  Silva, Láuris Lucia da³  Lopes, Júlio César Dias³  Santoro, Marcelo Matos³  Bravo, Carlos E. Salas³  Santos, Alexandre Martins Costa⁴  Montanari, Carlos Alberto⁵ 

[1] Centro Federal de Educação Tecnológica, Timóteo, Brasil;Universidade Federal de Alagoas, Maceió, Brasil;Universidade Federal de Minas Gerais, Belo Horizonte, Brasil;Universidade Federal do Espírito Santo, Vitória, Brasil;Universidade de São Paulo, São Carlos, Brasil

关键词: biscationic compounds and interaction with DNA;    isothermal titration calorimetry;    electrochemical biosensor.;   

DOI  :  10.1590/S0100-40422012000700007

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Biscationic amidines bind in the DNA minor groove and present biological activity against a range of infectious diseases. Two new biscationic compounds (bis-α,ω-S-thioureido, amino and sulfide analogues) were synthesized in good yields and fully characterized, and their interaction with DNA was also investigated. Isothermal titration calorimetry (ITC) was used to measure the thermodynamic properties of binding interactions between DNA and these ligands. A double stranded calf thymus DNA immobilized on an electrode surface was used to study the possible DNA-interacting abilities of these compounds towards dsDNA in situ. A remarkable interaction of these compounds with DNA was demonstrated and their potential application as anticancer agents was furthered.

【 授权许可】

CC BY