Electrochemical and calorimetric investigation of interaction of novel biscationic anticancer agents with DNA

Química Nova

Silva, Láuris Lucia da¹  Universidade Federal do Espírito Santo, Vitória, Brasil¹  Universidade de São Paulo, São Carlos, Brasil¹  Abreu, Fabiane Caxico de¹  Santoro, Marcelo Matos¹  Centro Federal de Educação Tecnológica, Timóteo, Brasil¹  Donnici, Claudio Luis¹  Santos, Alexandre Martins Costa¹  Paula, Francine Santos de¹  Denadai, Ângelo Márcio Leite¹  Universidade Federal de Alagoas, Maceió, Brasil¹  Montanari, Carlos Alberto¹  Goulart, Marília Oliveira Fonseca¹  Lopes, Júlio César Dias¹  Universidade Federal de Minas Gerais, Belo Horizonte, Brasil¹  Bravo, Carlos E. Salas¹ 

关键词: biscationic compounds and interaction with DNA;    isothermal titration calorimetry;    electrochemical biosensor.;   

DOI  :  10.1590/S0100-40422012000700007

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Biscationic amidines bind in the DNA minor groove and present biological activity against a range of infectious diseases. Two new biscationic compounds (bis-α,ω-S-thioureido, amino and sulfide analogues) were synthesized in good yields and fully characterized, and their interaction with DNA was also investigated. Isothermal titration calorimetry (ITC) was used to measure the thermodynamic properties of binding interactions between DNA and these ligands. A double stranded calf thymus DNA immobilized on an electrode surface was used to study the possible DNA-interacting abilities of these compounds towards dsDNA in situ. A remarkable interaction of these compounds with DNA was demonstrated and their potential application as anticancer agents was furthered.

【 授权许可】

Unknown