Development of a UV/Vis spectrophotometric method for analysis of total polyphenols from Caesalpinia peltophoroides Benth

Química Nova

Fernanda G. Bueno²  Maria A. D. Machareth²  Gean P. Panizzon²  Gisely C. Lopes²  João C. P. Mello²  Eneri V. S. Leite-mello¹ 

[1] ,Universidade Estadual de Maringá Departamento de Farmácia Maringá PR ,Brasil

关键词: Caesalpinia peltophoroides;    total polyphenols;    UV/VIS spectroscopy validation;   

DOI  :  10.1590/S0100-40422012000400031

来源: SciELO

【 摘 要 】

Caesalpinia peltophoroides is a domesticated tree found in Brazil. It was necessary to develop an analytical method to determine the content of total polyphenols (TP) in this herbal drug. The pre-analytical method was standardized for analysis time, wavelength, and the best standard to use. The optimum conditions were: pyrogallol, 760 nm, and 30 min respectively. Under these conditions, validation by UV/Vis spectrophotometry proved to be reliable for TP of the crude extract and semipurified fractions from C. peltophoroides. Standardization is required for every herbal drug, and this method proved to be linear, precise, accurate, reproducible, robust, and easy to perform.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License