Development of a UV/Vis spectrophotometric method for analysis of total polyphenols from Caesalpinia peltophoroides Benth

Química Nova

Lopes, Gisely C.¹  Leite-Mello, Eneri V. S.¹  Mello, João C. P.¹  Panizzon, Gean P.¹  Machareth, Maria A. D.¹  Bueno, Fernanda G.¹  Universidade Estadual de Maringá, Maringá, Brasil¹ 

关键词: Caesalpinia peltophoroides;    total polyphenols;    UV/VIS spectroscopy validation.;   

DOI  :  10.1590/S0100-40422012000400031

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Caesalpinia peltophoroides is a domesticated tree found in Brazil. It was necessary to develop an analytical method to determine the content of total polyphenols (TP) in this herbal drug. The pre-analytical method was standardized for analysis time, wavelength, and the best standard to use. The optimum conditions were: pyrogallol, 760 nm, and 30 min respectively. Under these conditions, validation by UV/Vis spectrophotometry proved to be reliable for TP of the crude extract and semipurified fractions from C. peltophoroides. Standardization is required for every herbal drug, and this method proved to be linear, precise, accurate, reproducible, robust, and easy to perform.

【 授权许可】

Unknown