Química Nova | |
Development of a UV/Vis spectrophotometric method for analysis of total polyphenols from Caesalpinia peltophoroides Benth | |
Fernanda G. Bueno2  Maria A. D. Machareth2  Gean P. Panizzon2  Gisely C. Lopes2  João C. P. Mello2  Eneri V. S. Leite-mello1  | |
[1] ,Universidade Estadual de Maringá Departamento de Farmácia Maringá PR ,Brasil | |
关键词: Caesalpinia peltophoroides; total polyphenols; UV/VIS spectroscopy validation; | |
DOI : 10.1590/S0100-40422012000400031 | |
来源: SciELO | |
【 摘 要 】
Caesalpinia peltophoroides is a domesticated tree found in Brazil. It was necessary to develop an analytical method to determine the content of total polyphenols (TP) in this herbal drug. The pre-analytical method was standardized for analysis time, wavelength, and the best standard to use. The optimum conditions were: pyrogallol, 760 nm, and 30 min respectively. Under these conditions, validation by UV/Vis spectrophotometry proved to be reliable for TP of the crude extract and semipurified fractions from C. peltophoroides. Standardization is required for every herbal drug, and this method proved to be linear, precise, accurate, reproducible, robust, and easy to perform.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202005130063904ZK.pdf | 315KB | download |