Purificação e caracterização de uma gentioexaose obtida de botriosferana por hidrólise ácida parcial

Química Nova

Iara Ribeiro Silva¹  Nilson Kobori Monteiro¹  Paula Felippe Martinez¹  Nataly Lino Izeli¹  Ana Flora Dalberto Vasconcelos¹  Marilsa De Stefani Cardoso¹  Maria De Lourdes Corradi Da Silva¹  Aneli De Melo Barbosa²  Robert F. H. Dekker²  Gil Valdo José Da Silva²  Luís Alberto Beraldo De Moraes² 

[1] ,Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências e Tecnologia Departamento de Física, Química e BiologiaPresidente Prudente SP ,Brasil

关键词: oligosaccharide;    partial acid hydrolysis;    β-glucan;   

DOI  :  10.1590/S0100-40422008000500013

来源: SciELO

【 摘 要 】

A hexa-oligosaccharide was obtained by partial acid hydrolysis from botryosphaeran, an exopolysaccharide (EPS) β(1→3; 1→6)-D-glucan type, produced by the ascomyceteous fungus Botryosphaeria rhodina. The oligosaccharide was purified by gel filtration and charcoal-Celite column chromatography and the analysis was followed by HPAEC/PAD. The structure was determined by NMR spectroscopy and mass spectrometry, which showed that the oligosaccharide consists of six β-D-glucopiranosyl units O-6 substituted (gentiohexaose).

【 授权许可】

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