Purification and characterization of a gentiohexaose obtained from botryosphaeran by partial acid hydrolysis

Química Nova

Dekker, Robert F. H.¹  Martinez, Paula Felippe¹  Silva, Maria de Lourdes Corradi da¹  Vasconcelos, Ana Flora Dalberto¹  Universidade Estadual Paulista Júlio de Mesquita Filho, Presidente Prudente, Brasil¹  Universidade Estadual de Londrina, Londrina, Brasil¹  Barbosa, Aneli de Melo¹  Cardoso, Marilsa de Stefani¹  Izeli, Nataly Lino¹  Universidade de São Paulo, Ribeirão Preto, Brasil¹  Universidad de Castilla-La Mancha, Ciudad Real, España¹  Silva, Iara Ribeiro¹  Silva, Gil Valdo José da¹  Monteiro, Nilson Kobori¹  Moraes, Luís Alberto Beraldo de¹ 

关键词: oligosaccharide;    partial acid hydrolysis;    β;    -;    glucan.;   

DOI  :  10.1590/S0100-40422008000500013

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A hexa-oligosaccharide was obtained by partial acid hydrolysis from botryosphaeran, an exopolysaccharide (EPS) β(1→3; 1→6)-D-glucan type, produced by the ascomyceteous fungus Botryosphaeria rhodina. The oligosaccharide was purified by gel filtration and charcoal-Celite column chromatography and the analysis was followed by HPAEC/PAD. The structure was determined by NMR spectroscopy and mass spectrometry, which showed that the oligosaccharide consists of six β-D-glucopiranosyl units O-6 substituted (gentiohexaose).

【 授权许可】

Unknown