Purification and characterization of a gentiohexaose obtained from botryosphaeran by partial acid hydrolysis

Quimica nova

Dekker, Robert F. H.¹  Martinez, Paula Felippe²  Izeli, Nataly Lino²  Cardoso, Marilsa de Stefani²  Silva, Maria de Lourdes Corradi da²  Silva, Iara Ribeiro²  Vasconcelos, Ana Flora Dalberto²  Monteiro, Nilson Kobori²  Barbosa, Aneli de Melo³  Silva, Gil Valdo José da⁴  Moraes, Luís Alberto Beraldo de⁴ 

[1] Universidad de Castilla-La Mancha, Ciudad Real, España;Universidade Estadual Paulista Júlio de Mesquita Filho, Presidente Prudente, Brasil;Universidade Estadual de Londrina, Londrina, Brasil;Universidade de São Paulo, Ribeirão Preto, Brasil

关键词: oligosaccharide;    partial acidhydrolysis;    β;    -;    glucan.;   

DOI  :  10.1590/S0100-40422008000500013

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A hexa-oligosaccharide was obtained by partialacid hydrolysis from botryosphaeran, an exopolysaccharide (EPS) β(1→3;1→6)-D-glucan type, produced by the ascomyceteous fungus Botryosphaeriarhodina. The oligosaccharide was purified by gel filtration and charcoal-Celitecolumn chromatography and the analysis was followed by HPAEC/PAD. The structurewas determined by NMR spectroscopy and mass spectrometry, which showed thatthe oligosaccharide consists of six β-D-glucopiranosyl units O-6substituted (gentiohexaose).

【 授权许可】

CC BY