Desenvolvimento e validação de metodologia analítica para determinação de itraconazol em produtos farmacêuticos por CLAE

Química Nova

Edith Cristina Laignier Cazedey¹  Roberta De Cássia Pimentel Azevedo¹  Érika De Fátima Silva¹  Magali Benjamim De Araújo¹ 

[1] ,Universidade Federal de Alfenas Departamento de Farmácia Alfenas MG ,Brasil

关键词: itraconazole;    validation;    HPLC;   

DOI  :  10.1590/S0100-40422007000400004

来源: SciELO

【 摘 要 】

Itraconazole is a synthetic antifungal drug administered orally with a broad spectrum of activity against mycotic infections. The present work consists of the development and validation of analytical methodology for evaluation of itraconazole in pharmaceutical products by high performance liquid chromatography. The separation was made using the reversed-phase column LC-18, acetonitrile/diethylamine 0.05% v/v, 60:40 v/v, pH 8.0 as mobile phase, methanol as solvent and detection and quantification at 254 nm. The results here obtained show that the analytical methodology is accurate, reproducible, robust and linear over the concentration range 8.0-12.0 µg/mL of itraconazole. The method was applied to pharmaceutical capsules containg itraconazole pellets and showed to be efficient, yielding good results.

【 授权许可】

CC BY-NC   
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