Oxidação de proteínas por oxigênio singlete: mecanismos de dano, estratégias para detecção e implicações biológicas

Química Nova

Graziella E. Ronsein²  Sayuri Miyamoto²  Etelvino Bechara²  Paolo Di Mascio²  Glaucia R. Martinez¹ 

[1] ,Universidade de São Paulo Instituto de Química Departamento de BioquímicaSão Paulo SP ,Brasil

关键词: singlet oxygen;    protein oxidation;    protein peroxide;   

DOI  :  10.1590/S0100-40422006000300027

来源: SciELO

【 摘 要 】

Proteins are potential targets for singlet molecular oxygen (¹O2) oxidation. Damages occur only at tryptophan, tyrosine, histidine, methionine, and cysteine residues at physiological pH, generating oxidized compounds such as hydroperoxides. Therefore, it is important to understand the mechanisms by which ¹O2, hydroperoxides and other oxidized products can trigger further damage. The improvement and development of new tools, such as clean sources of ¹O2 and isotopic labeling approaches in association with HPLC/mass spectrometry detection will allow one to elucidate mechanistic features involving ¹O2-mediated protein oxidation.

【 授权许可】

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