Singlet oxygen-mediated protein oxidation: damage mechanisms, detection techniques and biological implication

Quimica nova

Martinez, Glaucia R.¹  Di Mascio, Paolo²  Miyamoto, Sayuri²  Ronsein, Graziella E.²  Bechara, Etelvino² 

[1] Universidade Federal do Paraná, Curitiba, Brasil;Universidade de São Paulo, São Paulo, Brasil

关键词: singlet oxygen;    protein oxidation;    protein peroxide.;   

DOI  :  10.1590/S0100-40422006000300027

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Proteins are potentialtargets for singlet molecular oxygen (1O2) oxidation.Damages occur only at tryptophan, tyrosine, histidine, methionine, and cysteineresidues at physiological pH, generating oxidized compounds such as hydroperoxides.Therefore, it is important to understand the mechanisms by which 1O2,hydroperoxides and other oxidized products can trigger further damage. The improvementand development of new tools, such as clean sources of 1O2and isotopic labeling approaches in association with HPLC/mass spectrometrydetection will allow one to elucidate mechanistic features involving 1O2-mediatedprotein oxidation.

【 授权许可】

CC BY