Singlet oxygen-mediated protein oxidation: damage mechanisms, detection techniques and biological implication

Química Nova

Di Mascio, Paolo¹  Miyamoto, Sayuri¹  Ronsein, Graziella E.¹  Universidade de São Paulo, São Paulo, Brasil¹  Universidade Federal do Paraná, Curitiba, Brasil¹  Martinez, Glaucia R.¹  Bechara, Etelvino¹ 

关键词: singlet oxygen;    protein oxidation;    protein peroxide.;   

DOI  :  10.1590/S0100-40422006000300027

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Proteins are potential targets for singlet molecular oxygen (1O2) oxidation. Damages occur only at tryptophan, tyrosine, histidine, methionine, and cysteine residues at physiological pH, generating oxidized compounds such as hydroperoxides. Therefore, it is important to understand the mechanisms by which 1O2, hydroperoxides and other oxidized products can trigger further damage. The improvement and development of new tools, such as clean sources of 1O2 and isotopic labeling approaches in association with HPLC/mass spectrometry detection will allow one to elucidate mechanistic features involving 1O2-mediated protein oxidation.

【 授权许可】

Unknown