Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis

Memórias do Instituto Oswaldo Cruz

Gabriella Freitas Ferreira²  Cícero Moraes²  Alda Maria Soares Da Silveira²  Rodrigo Correa-oliveira¹  Andréa Teixeira-carvalho¹  Olindo Assis Martins-filho¹  Elizabeth Castro Moreno¹  Luiz Simeão Do Carmo¹  Lucia Alves De Oliveira Fraga²  Luiz Cosme Cotta Malaquias² 

[1] ,Universidade Vale do Rio Doce Núcleo de Pesquisa em Imunologia Governador Valadares MG ,Brasil

关键词: Staphylococcus aureus;    enterotoxin A;    chronic osteomyelitis;    cytokines;    IL-6;   

DOI  :  10.1590/S0074-02762012000300009

来源: SciELO

【 摘 要 】

We investigated the cytokine profile of peripheral mononuclear cells from chronic osteomyelitis (OST) patients following in vitro stimulation with staphylococcal enterotoxin A (SEA). We demonstrate that stimulation with SEA induced prominent lymphocyte proliferation and high levels of tumour necrosis factor (TNF)-α, interleukin (IL)-4 and IL-10 secretion in both OST and non-infected individuals (NI). Even though stimulation with SEA had no impact on IL-6 production in either patient group, the baseline level of IL-6 production by cells from OST patients was always significantly less than that produced by cells from NI. After classifying the osteomyelitic episodes based on the time after the last reactivation event as "early" (1-4 months) or "late" osteomyelitis (5-12 months), we found that increased levels of TNF-α and IL-4 in combination with decreased levels of IL-6 were observed in the early episodes. By contrast, increased levels of IL-10, IL-2 and IL-6 were hallmarks of late episodes. Our data demonstrate that early osteomyelitic episodes are accompanied by an increased frequency of "high producers" of TNF-α and IL-4, whereas late events are characterised by increased frequencies of "high producers" of IL-10, IL-6 and IL-2. These findings demonstrate the distinct cytokine profiles in chronic osteomyelitis, with a distinct regulation of IL-6 production during early and late episodes.

【 授权许可】

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