Benznidazole levels in blood vary with age in rats

Memórias do Instituto Oswaldo Cruz

Romina Fernanda Bulffer¹  José Alberto Castro¹  Silvia Laura Fanelli¹ 

[1] ,Institute of Scientific and Technological Research of the Armed Forces Centro de Investigaciones Toxicológicas Buenos Aires,Argentina

关键词: benznidazole;    rat blood;    HPLC;    Chagas disease;   

DOI  :  10.1590/S0074-02762011000300021

来源: SciELO

【 摘 要 】

Benznidazole (Bz) exhibits toxic side effects in animal studies and clinical use. Reductive metabolism of Bz in liver microsomes modulates the duration of its chemotherapeutic effect and its toxicity. The rate of this metabolism depends on age and is less intense in newborns and youngsters than in adults. In the present study, we determined Bz blood levels in rats of different ages that received Bz intragastrically (100 mg/kg). We developed and validated a high-pressure liquid chromatography with UV detector method for determination of Bz levels in whole blood. Bz levels were significantly higher and persisted for longer periods of time in the blood of young rats when compared to that of adult animals.

【 授权许可】

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