Adenoviruses C in non-hospitalized Mexican children older than five years of age with acute respiratory infection

Memórias do Instituto Oswaldo Cruz

Dora P Rosete²  María Eugenia Manjarrez¹  Blanca L Barrón² 

[1] ,Instituto Politécnico Naciona Escuela Nacional de Ciencias Biológicas Laboratorio de VirologíaMéxico D.F. ,México

关键词: PCR/RFLP;    adenovirus C;    ARI;    non-hospitalized children;   

DOI  :  10.1590/S0074-02762008000200012

来源: SciELO

【 摘 要 】

Adenoviruses (AdV) are commonly involved in acute respiratory infections (ARI), which cause high morbidity and mortality in children. AdV are grouped in six species (A-F), which are associated with a wide range of diseases. The aim of this study was to identify the AdV species infecting non-hospitalized Mexican children with ARI symptoms, attending to the same school. For that, a PCR/RFLP assay was designed for a region of the hexon gene, which was chosen, based on the bioinformatical analysis of AdV genomes obtained from GenBank. A total of 100 children's nasopharyngeal samples were collected from January to June, 2005, and used for viral isolation in A549 cells and PCR/RFLP analysis. Only 15 samples produced cytopathic effect, and in all of them AdV C was identified. AdV C was also identified in eight additional nasopharyngeal samples which were negative for viral isolation. In summary, this outpatient population showed a rate of AdV infection of 23%, and only AdV C was detected.

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