Reliable Sex Identification of Dogs by Modified PCR/RFLP Analysis

Journal of Veterinary Medical Science

Hideo FUJITANI³  Yong-Soon LEE¹  Chikako MATSUBA³  Masaru MURAKAMI³  Hiroshi FUJISE² 

[1] College of Veterinary Medicine, Seoul National University;Laboratory of Pathobiochemistry, Azabu University School of Veterinary Medicine;Laboratory of Molecular Biology, Azabu University School of Veterinary Medicine

关键词: Canis familialis;    PCR/RFLP;    sex determination;   

DOI  :  10.1292/jvms.63.679

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(12)Cited-By(5)To find definitive RFLP sites for canine sex determination, DNA segments corresponding to parts of the canine ZFX and ZFY genes were amplified by PCR and were directly sequenced. According to the newly defined sequence data, the combination of HaeIII and Cfr13I sites was found to be useful for not only identifying the sex of the canine DNA samples but also distinguishing them from the human DNA. Conveniently, these two enzymes worked simultaneously in the same single buffer. The double-digestion of the ZFX/ZFY PCR products with HaeIII and Cfr13I showed banding patterns unique to males and females in Canis familialis. This PCR/RFLP method was confirmed to be applicable to various breeds of dog.

【 授权许可】

Unknown