Description of an in vivo model for the assessment of eosinophil chemoattractants in the mouse

Memórias do Instituto Oswaldo Cruz

Mauro M Teixeira¹  Timothy J Williams¹  Paul G Hellewell¹ 

[1] ,National Heart and Lung Institute Imperial College of Medicine Applied PharmacologyLondon

关键词: chemokines;    eosinophils;    eotaxin;    recruitment;   

DOI  :  10.1590/S0074-02761997000800029

来源: SciELO

【 摘 要 】

Chemokines (chemoattractant cytokines) induce potent and selective chemotaxis of leukocyte subsets in vitro. Here, we review briefly the chemokines shown to induce eosinophil chemotaxis in vitro and describe a novel model for the study of the ability of chemokines to stimulate eosinophil migration in vivo. Eosinophils were purified from the blood of mice over-expressing the IL-5 gene and labelled with 111In. Only the C-C chemokines, eotaxin and MIP-1alpha, but not RANTES, MCP-1, MCP-3, MCP-4, MIP-1ß, KC and MIP-2, effectively induced the recruitment of 111In-eosinophils in mouse skin. We suggest that this mouse model will be useful in assessing the role of endogenously-generated chemokines in mediating eosinophil migration to sites of allergic inflammation in vivo.

【 授权许可】

CC BY   
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