Atividades citotóxica e hemolítica em Escherichia coli uropatogênicas

Memórias do Instituto Oswaldo Cruz

João Ramos Costa Andrade¹  Ítalo Suassuna¹ 

[1] ,UERJ Faculdade de Ciências Médicas Serviço de Microbiologia e ImunologiaRio de Janeiro,Brasil

关键词: Escherichia coli;    urinary infections;    cytotoxins;    hemolysins;    uropathogenicity;    Escherichia coli;    infecções urinárias;    citotoxinas;    hemolisinas;    uropatogenicidade;   

DOI  :  10.1590/S0074-02761988000200008

来源: SciELO

【 摘 要 】

Fifty nine Escherichia coli strains obtained from patients with upper or lower urinary tract infections (UTI) and 30 E. coli strains isolated from stools of healthy individuals were tested for hemolytic and totoxic activities. Forty four percent of uropathologenic e. coli (UPEC) and 3.3% of fecal E. coli were hemolytic. Among the hemolytic UPEC, 92% produced x-hemolysin. A cytotoxic activity was detected in culture filtrates of 71% of UPEC strains and 30% of fecal E. coli. No relationship was found between cytotoxic and hemolytic activities or between cytotoxic titers and UPEC origin (upper or lower UTI). E. coli cytotoxin has a cytocidal activity against some epithelioid cultured cell lines (Vero, HeLa and Hep-2) but was almost inactive for avian-fibroblast cells. Cytotoxin-affected cells appeared rounded, refractile and detached from the surface of the vessel. Some characteristics exhibited by the cytotoxin as the morphological response induced on cells, the increasing of cytopathic effect with time, its irreversible cytocidal activity and its heat-lability resemble the properties described for E. coli Verotoxin (VT). Adherence to uroepithelial cells is recognized as a virulence factor for UPEC. It is suggested that cell damage by cytotoxic and adhering UPEC might contribute to E. coli virulence to urinary tract.

【 授权许可】

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