Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Vlademir Cantarelli²  Bianca Cavalcante²  Diogo André Pilger²  Fabiana Souza²  Cícero Gomes Dias¹  Teresa Brodt²  Maritza Cantarelli¹  Carina Secchi²  Pedro Alves D'azevedo¹ 

[1] ,Weinmann Laboratório

关键词: RT-PCR;    Vancomycin resistant enterococci;    Genes vanA-vanB;    RT-PCR;    Enterococos resistentes à vancomicina;    Genes vanA-vanB;   

DOI  :  10.1590/S0037-86822011000500021

来源: SciELO

【 摘 要 】

INTRODUCTION: Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE). METHODS: The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program. RESULTS: Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectively) when compared to broth enriched culture, whereas specificity was 83.1%. CONCLUSIONS: RT-PCR provides same day results, however it showed low sensitivity for VRE detection.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License