Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Brodt, Teresa¹  d'Azevedo, Pedro Alves¹  Hospital Moinhos de Vento, Porto Alegre¹  Dias, Cícero Gomes¹  Cavalcante, Bianca¹  Weinmann Laboratório¹  Cantarelli, Vlademir¹  Universidade Federal de Ciências da Saúde de Porto Alegre¹  Cantarelli, Maritza¹  Pilger, Diogo André¹  Souza, Fabiana¹  Secchi, Carina¹ 

关键词: RT-PCR;    Vancomycin resistant enterococci;    Genes vanA-vanB;   

DOI  :  10.1590/S0037-86822011000500021

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION: Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE).METHODS: The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program.RESULTS: Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectively) when compared to broth enriched culture, whereas specificity was 83.1%.CONCLUSIONS: RT-PCR provides same day results, however it showed low sensitivity for VRE detection.

【 授权许可】

Unknown