A semi-nested PCR assay for molecular detection of Paracoccidioides brasiliensis in tissue samples

Revista da Sociedade Brasileira de Medicina Tropical

Andrea Cristine Koishi²  Débora Fonseca Vituri²  Pedro Sebastião Raimundo Dionízio Filho²  Alexandre Augusto Sasaki²  Maria Sueli Soares Felipe¹  Emerson José Venancio² 

[1] ,Londrina State University Department of Pathological Sciences Program in Experimental PathologyLondrina PR ,Brazil

关键词: Paracoccidioides brasiliensis;    Semi-nested PCR;    Molecular detection;    Paracoccidioides brasiliensis;    Semi-nested PCR;    Detecção molecular;   

DOI  :  10.1590/S0037-86822010000600026

来源: SciELO

【 摘 要 】

INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction, while the primers MJ03 and ITS1 primer were used in the second reaction. The semi-nested PCR was used to investigate biopsies of five patients with oral lesions that resembled paracoccidioidomycosis. RESULTS: The semi-nested PCR was positive for four samples and negative for a sample from a patient later diagnosed with leishmaniasis. CONCLUSIONS: The new semi-nested PCR describe is useful for paracoccidioidomycosis diagnosis.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License