A semi-nested PCR assay for molecular detection of Paracoccidioides brasiliensis in tissue samples

Revista da Sociedade Brasileira de Medicina Tropical

Dionízio Filho, Pedro Sebastião Raimundo¹  Sasaki, Alexandre Augusto¹  Vituri, Débora Fonseca¹  Brasilia University, Brasília, Brazil¹  Londrina State University, Londrina, Brazil¹  Felipe, Maria Sueli Soares¹  Koishi, Andrea Cristine¹  Venancio, Emerson José¹ 

关键词: Paracoccidioides brasiliensis;    Semi-nested PCR;    Molecular detection;   

DOI  :  10.1590/S0037-86822010000600026

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis.METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction, while the primers MJ03 and ITS1 primer were used in the second reaction. The semi-nested PCR was used to investigate biopsies of five patients with oral lesions that resembled paracoccidioidomycosis.RESULTS: The semi-nested PCR was positive for four samples and negative for a sample from a patient later diagnosed with leishmaniasis.CONCLUSIONS: The new semi-nested PCR describe is useful for paracoccidioidomycosis diagnosis.

【 授权许可】

Unknown