Viability and molecular authentication of Coccidioides immitis strains from culture collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Claudia C.f. Bezerra²  Renata F. De Lima¹  Márcia S. Lazera²  Bodo Wanke²  Cíntia M. Borba¹ 

[1] ,Fundação Oswaldo Cruz Instituto de Pesquisa Clínica Evandro Chagas Serviço de MicologiaRio de Janeiro RJ

关键词: Coccidioides immitis;    Long-term preservation;    PCR;    Coccidioides immitis;    Preservação por longos períodos;    PCR;   

DOI  :  10.1590/S0037-86822006000300002

来源: SciELO

【 摘 要 】

Twenty Coccidioides immitis strains were evaluated. Only 5 of the 20 strains kept under mineral oil maintained their viability while all 5 subcultures preserved in water remained viable and none of the 13 subcultures kept in soil were viable. A 519 bp PCR product from the csa gene confirmed the identity of the strains.

【 授权许可】

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