Viability and molecular authentication of Coccidioides immitis strains from culture collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Wanke, Bodo¹  Lima, Renata F. de¹  Lazera, Márcia S.¹  Borba, Cíntia M.¹  Bezerra, Claudia C.F.¹  Fundação Oswaldo Cruz, Rio de Janeiro¹ 

关键词: Coccidioides immitis;    Long-term preservation;    PCR;   

DOI  :  10.1590/S0037-86822006000300002

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

Twenty Coccidioides immitis strains were evaluated. Only 5 of the 20 strains kept under mineral oil maintained their viability while all 5 subcultures preserved in water remained viable and none of the 13 subcultures kept in soil were viable. A 519 bp PCR product from the csa gene confirmed the identity of the strains.

【 授权许可】

Unknown