Influence of canine brain decomposition on laboratory diagnosis of rabies

Revista da Sociedade Brasileira de Medicina Tropical

Avelino Albas²  Clara Izabel De Lucca Ferrari¹  Luzia Helena Queiroz Da Silva¹  Fernanda Bernardi¹  Fumio Honma Ito¹ 

[1] ,Instituto BiológicoPresidente Prudente SP ,Brasil

关键词: Immunofluorescence;    Rabies;    Mice inoculation;    Decomposition;    Canine brain;    Imunofluorescência;    Raiva;    Inoculação em camundongos;    Decomposição;   

DOI  :  10.1590/S0037-86821999000100004

来源: SciELO

【 摘 要 】

Canine brains infected with rabies virus were submitted to decomposition by being left at room temperature of 25 to 29oC for up to 168h. At 24h intervals, brain fragments were analyzed by immunofluorescence (IF) and by the mouse intracerebral inoculation (MI) test to confirm the diagnosis of rabies and to measure the putrefaction effect on the accuracy of the diagnosis. Forty eight h after the beginning of the experiment, the MI test showed signs of impairment with four negative results, while after 72h, 100% of the results were negative to the MI test and only one result was negative to the IF test, indicating that the threshold period for accurate diagnosis is 24 to 48h before putrefaction. The authors recommend the shipment of suspected cases of rabies to the laboratory for confirmation, but the use of putrid materials for diagnosis is meaningless because of false-negative results.

【 授权许可】

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