Revista da Sociedade Brasileira de Medicina Tropical | |
Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans | |
Jesús M. Egido1  Juan Viñuelas1  | |
[1] ,Santiago University,Spain | |
关键词: Phagocytosis; Flow cytometry; Latex particles; Candida albicans; Fagocitosis; Flujo citométrico; Partículas de látex; Candida albicans; | |
DOI : 10.1590/S0037-86821997000600001 | |
来源: SciELO | |
【 摘 要 】
We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripherial blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1µm diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripherial blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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