Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans

Revista da Sociedade Brasileira de Medicina Tropical

Jesús M. Egido¹  Juan Viñuelas¹ 

[1] ,Santiago University,Spain

关键词: Phagocytosis;    Flow cytometry;    Latex particles;    Candida albicans;    Fagocitosis;    Flujo citométrico;    Partículas de látex;    Candida albicans;   

DOI  :  10.1590/S0037-86821997000600001

来源: SciELO

【 摘 要 】

We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripherial blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1µm diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripherial blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB.

【 授权许可】

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