ANTHELMINTIC ACTIVITY OF LAPACHOL, β-LAPACHONE AND ITS DERIVATIVES AGAINST Toxocara canis LARVAE

Revista do Instituto de Medicina Tropical de São Paulo

Taís Mata-santos¹  Nitza França Pinto¹  Hilton Antônio Mata-santos¹  Kelly Gallan De Moura¹  Paula Fernandes Carneiro¹  Tatiane Dos Santos Carvalho¹  Karina Pena Del Rio¹  Maria Do Carmo Freire Ribeiro Pinto¹  Lourdes Rodrigues Martins¹  Juliana Montelli Fenalti¹  Pedro Eduardo Almeida Da Silva¹  Carlos James Scaini¹ 

关键词: Toxocara canis;    Quinones;    Chemotherapy;    Anthelmintics;   

DOI  :  10.1590/S0036-46652015000300003

来源: SciELO

【 摘 要 】

Anthelmintics used for intestinal helminthiasis treatment are generally effective; however, their effectiveness in tissue parasitosis (i.e. visceral toxocariasis) is moderate. The aim of this study was to evaluate the in vitroactivity of lapachol, β-lapachone and phenazines in relation to the viability of Toxocara canis larvae. A concentration of 2 mg/mL (in duplicate) of the compounds was tested using microculture plates containing Toxocara canis larvae in an RPMI-1640 environment, incubated at 37 °C in 5% CO2 tension for 48 hours. In the 2 mg/mL concentration, four phenazines, lapachol and three of its derivatives presented a larvicide/larvistatic activity of 100%. Then, the minimum larvicide/larvistatic concentration (MLC) test was conducted. The compounds that presented the best results were nor-lapachol (MLC, 1 mg/mL), lapachol (MLC 0.5 mg/mL), β-lapachone, and β-C-allyl-lawsone (MLC, 0.25 mg/mL). The larvae exposed to the compounds, at best MLC with 100% in vitro activity larvicide, were inoculated into healthy BALB/c mice and were not capable of causing infection, confirming the larvicide potential in vitro of these compounds.

【 授权许可】

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