First isolation of dengue 4 in the state of São Paulo, Brazil, 2011

Revista do Instituto de Medicina Tropical de São Paulo

Iray Maria Rocco¹  Vivian Regina Silveira¹  Adriana Yurika Maeda¹  Sarai Joaquim Dos Santos Silva¹  Carine Spenassatto¹  Ivani Bisordi¹  Akemi Suzuki¹ 

[1] ,Instituto Adolfo Lutz Núcleo de Doenças de Transmissão Vetorial São Paulo SP ,Brazil

关键词: Dengue 4;    Virus isolation;    RT-PCR;   

DOI  :  10.1590/S0036-46652012000100009

来源: SciELO

【 摘 要 】

We report the first isolation of Dengue virus 4 (DENV-4) in the state of São Paulo, from two patients - one living in São José do Rio Preto and the other one in Paulo de Faria, both cities located in the Northwest region of the state. The virus isolations were accomplished in the clone C6/36 Aedes albopictus cell line, followed by indirect immunofluorescence assays, performed with type-specific monoclonal antibodies that showed positive reactions for DENV-4. The results were confirmed by Nested RT-PCR and Real-Time RT-PCR assays. The introduction of DENV-4 in a country that already has to deal with the transmission of three other serotypes increases the possibility of the occurrence of more severe cases of the disease. The importance of early detection of dengue cases, before the virus spreads and major outbreaks occur, should be emphasized.

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