Identification of Cryptosporidium spp. oocysts in fecal smears stained with Heidenhain's iron hematoxylin

Revista do Instituto de Medicina Tropical de São Paulo

Cláudio Santos Ferreira¹  Vicente Amato Neto¹  Ruth Semira Rodríguez AlarcÓn¹  Erika Gakiya¹ 

[1] ,Universidade de São Paulo Faculdade de Medicina Laboratório de Investigação Médica/ParasitologiaSão Paulo,Brasil

关键词: Cryptosporidium spp.;    Regressive iron hematoxylin staining;   

DOI  :  10.1590/S0036-46652001000600009

来源: SciELO

【 摘 要 】

There is no paucity of methods for diagnosing Cryptosporidium spp. infection. The merits of immunoassays notwithstanding, microscopic identification of Cryptosporidium spp. oocysts in fecal samples remains an important diagnostic procedure. It owes the persistence of its use to such characteristics as dispensing with expensive equipment and kits, requiring only basic laboratory facilities, and having a low probability of false positive results when permanent slides are prepared, which can be re-examined in case of doubt. Cryptosporidium spp. oocysts can be readily identified in fecal smears prepared according to a regressive iron hematoxylin staining technique. The number of steps and their duration, as well as costs, were reduced to a minimum without loss of image quality and permanence of the preparations.

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