Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples

Revista do Instituto de Medicina Tropical de São Paulo

Elenice M.n. Gonçalves²  Ronalda S. Araújo¹  Magali Orban²  Glavur R. Matté¹  Maria Helena Matté¹  Carlos E.p. Corbett¹ 

[1] ,Universidade de São Paulo Faculdade de Medicina Hospital das ClínicasSão Paulo SP ,Brazil

关键词: Cryptosporidium spp.;    Diagnosis;    Molecular techniques;   

DOI  :  10.1590/S0036-46652008005000002

来源: SciELO

【 摘 要 】

Molecular characterization of Cryptosporidium spp.oocysts in clinical samples is useful for public health since it allows the study of sources of contamination as well as the transmission in different geographical regions. Although widely used in developed countries, in Brazil it is restricted to academic studies, mostly using commercial kits for the extraction of genomic DNA, or in collaboration with external reference centers, rendering the method expensive and limited. The study proposes the application of the modifications recently introduced in the method improving feasibility with lower cost. This method was efficient for clinical samples preserved at -20 °C for up to six years and the low number of oocysts may be overcomed by repetitions of extraction.

【 授权许可】

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