Single Spore Isolation as a Simple and Efficient Technique to obtain fungal pure culture

4th International Conference on Civil and Environmental Engineering for Sustainability

地球科学;生态环境科学;经济学

Noman, E.^1 ; Al-Gheethi, A.A.^2 ; Rahman, N.K.^3 ; Talip, B.^1 ; Mohamed, R.^2 ; H, N.^4 ; Kadir, O.A.^5

Faculty of Applied Sciences and Technology (FAST), Universiti Tun Hussein Onn Malaysia (UTHM), Pagoh, Johor, Malaysia^1

Micro-pollution Research Centre (MPRC), Department of Water and Environmental Engineering, Faculty of Civil and Environmental Engineering, Universiti Tun Hussein Onn Malaysia (UTHM), Parit Raja, Johor, Batu Pahat

86400, Malaysia^2

School of Distance Education, Universiti Sains Malaysia, Penang

11800, Malaysia^3

School of Biological Science, Universiti Sains Malaysia, Penang

11800, Malaysia^4

School of Industrial Technology, Universiti Sains Malaysia, Penang

11800, Malaysia^5

关键词: Clinical waste;    Culture medium;    Molecular techniques;    Pure culture;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/140/1/012055/pdf
DOI  :  10.1088/1755-1315/140/1/012055

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The successful identification of fungi by phenotypic methods or molecular technique depends mainly on the using an advanced technique for purifying the isolates. The most efficient is the single spore technique due to the simple requirements and the efficiency in preventing the contamination by yeast, mites or bacteria. The method described in the present work is depends on the using of a light microscope to transfer one spore into a new culture medium. The present work describes a simple and efficient procedure for single spore isolation to purify of fungi recovered from the clinical wastes.