Light-induced retinal injury enhanced neurotrophins secretion and neurotrophic effect of mesenchymal stem cells in vitro

Arquivos Brasileiros de Oftalmologia

Wei Xu¹  Xiaoting Wang¹  Guoxing Xu¹  Jian Guo¹ 

[1] ,Fujian Medical University First Affiliated Hospital Department of OphthalmologyFuzhou City,China

关键词: Fibroblast growth factor 2;    Ciliary neurotrophic factor;    Retina;    Mesenchymal stromal cells;    Stem cells;    Brain-derived neurotrophic factor;    Fator 2 de crescimento de fibroblastos;    Fator neurotrófico ciliar;    Retina;    Células mesenquimais estromais;    Células-tronco;    Fator neurotrófico derivado do encéfalo;   

DOI  :  10.1590/S0004-27492013000200010

来源: SciELO

【 摘 要 】

PURPOSE: To investigate neurotrophins expression and neurotrophic effect change in mesenchymal stem cells (MSCs) under different types of stimulation. METHODS: Rats were exposed in 10,000 lux white light to develop light-induced retinal injury. Supernatants of homogenized retina (SHR), either from normal or light-injured retina, were used to stimulate MSCs. Quantitative real time for polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were conducted for analysis the expression change in basic fibroblast growth factor (bFGF), brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) in MSCs after stimulation. Conditioned medium from SHR-stimulated MSCs and control MSCs were collected for evaluation their effect on retinal explants. RESULTS: Supernatants of homogenized retina from light-injured rats significantly promoted neurotrophins secretion from MSCs (p<0.01). Conditioned medium from mesenchymal stem cells stimulated by light-injured SHR significantly reduced DNA fragmentation (p<0.01), up-regulated bcl-2 (p<0.01) and down-regulated bax (p<0.01) in retinal explants, displaying enhanced protective effect. CONCLUSIONS: Light-induced retinal injury is able to enhance neurotrophins secretion from mesenchymal stem cells and promote the neurotrophic effect of mesenchymal stem cells.

【 授权许可】

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