Evaluation of epithelial cell proliferating activity and fibroblast nuclear kariometry in recurrent pterygium treated with mitomycin C

Arquivos Brasileiros de Oftalmologia

Gildasio Castello De Almeida Junior¹  Fabio Batista Frederico²  Karina Paula Watanabe²  Tatiana Vanucci Garcia²  Ângela Yuri Iquejiri²  Patrícia Maluf Cury²  Sebastião Roberto Taboga²  Reinaldo Azoubel² 

[1] ,Faculdade de Medicina de São José do Rio PretoSão José do Rio Preto SP ,Brasil

关键词: Ki-67 antigen;    Pterygium;    Recurrence;    Mitomycin;    Epithelial cells;    Fibroblasts;    Karyometry;    Antígeno Ki-67;    Pterígio;    Recidiva;    Mitomicina;    Células epiteliais;    Fibroblastos;    Cariometria;   

DOI  :  10.1590/S0004-27492008000400019

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the effectiveness of mitomycin C (MMC) in preventing recurrence of pterygium following conjunctival autograft transplantation (CAT). Ki-67 antigen to evaluate epithelial cell proliferation and fibroblast nuclear kariometry were used to assist treatment evaluation. METHODS: Twenty-nine patients with recurrent pterygium were divided into three groups: Group (G) 1 - CAT and placebo eyedrops (PED); G2 - CAT, 0.015% MMC subconjunctivally, and PED; G3 - CAT and 0.02% MMC eyedrops. Immunohistochemistry for the Ki-67 antigen and fibroblast nuclei kariometry were performed on the excised tissue, divided into nasal and temporal sides. Kariometry was evaluated in terms of volume (Vl) and area (Ar) using at least 50 cells/patient. RESULTS: The percentage of positive epithelial cells for the Ki-67 antigen on the nasal and temporal side after treatment of the three groups were: nasal (5.39% G1, 4.49% G2, and 3.88% G3); temporal (3.30% G1, 4.46% G2, 4.14% G3), did not show significant differences. Fibroblast nucleus kariometry was: nasal Vl (792.1 µ3 G1, 605.1 µ3 G2, and 549.9 µ3 G3) and Ar (100.58 µ2 G1, 83.13 µ2 G2, and 78.41 µ2 G3). The three groups showed significant differences: p=0.039 and p=0.035, for respectively Vl and Ar, on the nasal side. After a six month of treatment, the three groups presented the following recurrence rates: G1, 22.22%, G2, 18.18% and G3, 33.33%, respectively. CONCLUSION: MMC did not reduce the number of positive epithelial cells for the Ki-67 antigen in recurrent pterygium, but decreased fibroblast nucleus volume and area on the nasal side of the pterygia. The number of positive epithelial cells for the Ki-67 antigen seemed not to be related to pterygium recurrence observed over a six-month post-surgery period. The role of epithelial cell proliferation in pterygium recurrence should be evaluated by further studies.

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