Molecular characterization of Enterobacteriaceae resistant to carbapenem antimicrobials

Jornal Brasileiro de Patologia e Medicina Laboratorial

Jaciane B. Marques¹  Pauline C. Bonez¹  Vanessa A. Agertt¹  Vanessa C. Flores¹  Tanise V. Dalmolin¹  Grazielle G. Rossi¹  Nara Lucia F. Dal Forno¹  Bianca V. Bianchini¹  Caren R. Mizdal¹  Fallon S. Siqueira¹  Roselene A. Righi¹  Bettina H. Meneghetti¹  Priscila A. Trindade¹  Roberto C. V. Santos¹  Marli M. A. Campos¹ 

关键词: Enterobacteriaceae;    carbapenemase;    phenotypic methods;    molecular methods;    enterobactérias;    carbapenemases;    métodos fenotípicos;    métodos moleculares;   

DOI  :  10.5935/1676-2444.20150028

来源: SciELO

【 摘 要 】

ABSTRACT The present study aimed to genotypically and phenotypically characterize clinical isolates of carbapenem-resistant Enterobacteriaceae collected from inpatients at the University Hospital of Santa Maria, during seven months. Among the clinical isolates subjected to the modified Hodge test (MHT), 62.5% were positive, indicating possible production of carbapenemase. Polymerase chain reaction (PCR) demonstrated that blaKPC was the most frequently found gene (31%), followed by blaIMP (12.5%). Combined use of the methods is needed to identify carbapenem resistance in enterobacteria to prevent their spread and control the infections caused by these organisms.

【 授权许可】

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