期刊论文详细信息
Antibiotics
The Gut of Healthy Infants in the Community as a Reservoir of ESBL and Carbapenemase-Producing Bacteria
Alondra Diaz1  Patrick C. Seed1  Najeeb Rahman2  Aneeta Hotwani2  Anita K. Zaidi2  Shahida M. Qureshi2  Ali F. Saleem2  Ahreen Allana2  Asia Khan2  Lauren Hale3  Cristina Salinas3  Raul Salinas3  Mehreen Arshad3 
[1] Ann & Robert H. Lurie Children’s Hospital, 225 E Chicago Ave, Chicago, IL 60611, USA;Department of Pediatrics and Child Health, Aga Khan University Hospital, National Stadium Rd, Karachi 74800, Pakistan;Department of Pediatrics, Duke University, 2301 Erwin Rd, Durham, NC 27710, USA;
关键词: Enterobacteriaceae;    multi-drug resistant;    extended-spectrum beta-lactamase;    carbapenemase;    infants;    gut colonization;   
DOI  :  10.3390/antibiotics9060286
来源: DOAJ
【 摘 要 】

The recent rapid rise of multi-drug resistant Enterobacteriaceae (MDR-E) is threatening the treatment of common infectious diseases. Infections with such strains lead to increased mortality and morbidity. Using a cross-sectional study, we aimed to estimate the prevalence of gut colonization with extended spectrum beta-lactamase (ESBL) producing Enterobacteriaceae among healthy infants born in Pakistan, a setting with high incidence of MDR-E infections. Stool samples were collected from 104 healthy infants between the ages of 5 and 7 months. Enterobacteriaceae isolates were screened for resistance against several antimicrobial classes. Presence of ESBL and carbapenemase genes was determined using multiplex PCR. Sequence types were assigned to individual strains by multi-locus sequence typing. Phylogenetic analysis of Escherichia coli was done using the triplex PCR method. Forty-three percent of the infants were positive for ESBL-producing Enterobacteriaceae, the majority of which were E. coli. We identified several different ESBL E. coli sequence types most of which belonged to the phylogenetic group B2 (23%) or D (73%). The widespread colonization of infants in a developing country with ESBL-producing Enterobacteriaceae is concerning. The multiple sequence types and reported non-human sources support that multiple non-epidemic MDR lineages are circulating in Pakistan with healthy infants as a common reservoir.

【 授权许可】

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