A comparison between the novel rabbit monoclonal antibodies (SP1 and B644) and mouse antibodies for evaluating estrogen receptor in breast tumors

Jornal Brasileiro de Patologia e Medicina Laboratorial

Rafael Malagoli Rocha¹  Cristiana Buzelin Nunes¹  Gislene Fátima Silva Rocha²  Flávio Nepomuceno Oliveira²  Fernanda Squárcio Fernandes Sanches²  Helenice Gobbi² 

[1] ,Universidade Federal de Minas Gerais post-graduation program in pathology

关键词: Monoclonal antibodies;    Immunohistochemistry;    Breast cancer;    Anticorpos monoclonais;    Imuno-histoquímica;    Câncer de mama;   

DOI  :  10.1590/S1676-24442007000600008

来源: SciELO

【 摘 要 】

BACKGROUND: A novel generation of rabbit monoclonal antibodies has been released recently for estrogen (ER) and progesterone (PR) receptor evaluation in breast cancer by immunohistochemistry. Aims: We compared novel rabbit monoclonal antibodies anti-ER SP1 (LabVision®) and B644 (Cell Marque®) to mouse monoclonal antibodies 1D5 (Dako®) and 6F11 (Novocastra®) using a tissue microarray of breast carcinomas. METHODS: Two cylinders (2 mm diameter) of formalin-fixed paraffin embedded tissue were obtained from 24 invasive breast carcinomas and immunostained by using the anti-ER rabbit and mouse antibodies and the streptavidin-biotin detection system (Biogenex®). Immunostaining was evaluated considering positive those tumors in which more than 10% of the tumor cell nuclei stained. The stain intensity was also evaluated as weak (1), moderate (2), and strong (3). Results: Both rabbit antibodies against ER have similar staining pattern to each other and also to 6F11, but significantly stronger scores compared to mouse 1D5. The rabbit antibodies allow better cost/benefit because of higher working dilutions compared to mouse antibodies using the same procedure. CONCLUSION: The new rabbit antibodies against ER are highly sensitive and reliable in clinical and research immunohistochemical testing of breast carcinomas.

【 授权许可】

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