Whole cells in enantioselective reduction of benzyl acetoacetate

Brazilian Journal of Microbiology

Joyce Benzaquem Ribeiro²  Aline De Souza Ramos¹  Raquel De Oliveira Lopes²  Gabriela Veloso Vieira Da Silva²  Rodrigo Octavio Mendonça Alves De Souza² 

[1] ,Universidade Federal do Rio de Janeiro Instituto de Química Rio de Janeiro RJ ,Brazil

关键词: biotransformation;    chiral reduction;    β-ketoester;    Kluyveromyces marxianus;    immobilization;   

DOI  :  10.1590/S1517-83822014000300023

来源: SciELO

【 摘 要 】

The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction.

【 授权许可】

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