Whole cells in enantioselective reduction of benzyl acetoacetate

Revista de microbiologia

Ramos, Aline de Souza¹  Ribeiro, Joyce Benzaquem² 

[1] Fundação Oswaldo Cruz, Rio de Janeiro, Brazil;Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

关键词: biotransformation;    chiral reduction;    β;    -ketoester;    Kluyveromyces marxianus;    immobilization.;   

DOI  :  10.1590/S1517-83822014000300023

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction.

【 授权许可】

CC BY-NC