Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

Brazilian Journal of Microbiology

C.a.g. Leal¹  G.a. Carvalho-castro¹  A.c. Cottorello²  R.c. Leite¹  H.c.p. Figueiredo¹ 

[1],Universidade Federal de Minas Gerais Escola de Veterinária Laboratório de Doenças de Animais AquáticosBelo Horizonte MG ,Brazil

关键词: clinical samples;    sensitivity;    real-time PCR;    WSSV;    WSD;   

DOI  :  10.1590/S1517-83822013005000054

来源: SciELO

【 摘 要 】

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.

【 授权许可】

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