Biological control of rice brown spot with native isolates of three Trichoderma species

Brazilian Journal of Microbiology

Elham Khalili²  Mehdi Sadravi¹  Shahram Naeimi¹  Vahid Khosravi¹ 

[1] ,Gorgan University of Agriculture Science and Natural Resources Department of Plant Pathology Gorgan Golestan ,Iran

关键词: Rice brown spot;    Biocontrol;    Trichoderma harzianum;    T. virens;    T. atroviride;   

DOI  :  10.1590/S1517-83822012000100035

来源: SciELO

【 摘 要 】

Brown spot caused by Bipolaris oryzae is an important rice disease in Southern coast of Caspian Sea, the major rice growing region in Iran. A total of 45 Trichoderma isolates were obtained from rice paddy fields in Golestan and Mazandaran provinces which belonged to Trichoderma harzianum, T. virens and T. atroviride species. Initially, they were screened against B. oryzae by antagonism tests including dual culture, volatile and nonvolatile metabolites and hyperparasitism. Results showed that Trichoderma isolates can significantly inhibit mycelium growth of pathogen in vitro by producing volatile and nonvolatile metabolites Light microscopic observations showed no evidence of mycoparasitic behaviour of the tested isolates of Trichoderma spp. such as coiling around the B. oryzae. According to in vitro experiments, Trichoderma isolates were selected in order to evaluate their efficacy in controlling brown spot in glasshouse using seed treatment and foliar spray methods. Concerning the glasshouse tests, two strains of T. harzianum significantly controlled the disease and one strain of T. atroviride increased the seedling growth. It is the first time that the biological control of rice brown spot and increase of seedling growth with Trichoderma species have been studied in Iran.

【 授权许可】

CC BY-NC   
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