Records of Culturable Endophytic Fungi Inhabiting Rhizome of Elettaria in Hutan Sibayak, North Sumatera

4th International Conference on Biological Sciences and Biotechnology

Munir, E.^1 ; Lutfia, A.^1 ; Yurnaliza^1

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Kampus USU, Jalan Bioteknologi No. 1, Medan

20155, Indonesia^1

关键词: Fungal endophytes;    Molecular identification;    Neighbor joining;    Phylogenetic trees;    Schizophyllum commune;    Species specifics;    Trichoderma atroviride;    Trichoderma harzianum;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/305/1/012004/pdf
DOI  :  10.1088/1755-1315/305/1/012004

来源: IOP

【 摘 要 】

Endophytic microorganisms are microbial associates living in various part of host, yet expressing benefits to healthy plants. Existence of species-specific endophytes, endophytic fungi is still poorly studied especially in Zingiberaceae. In this study, we reported several endophytic fungal species isolated from rhizome of Elettaria through isolative efforts and molecular evidence. Host plant, Elettaria sp. was sampled from representative natural area, i.e. Hutan Sibayak which is known as biodiversity spot for Zingiberaceae in North-Sumatera. Molecular identification revealed the identity of five isolated fungal strains collected from rhizomes of Elettaria, namely Trichoderma atroviride, Curvularia lunata, Schizophyllum commune, Trichoderma harzianum and Pholiota multicingulata. Phylogenetic tree is constructed based on Neighbor-joining method in a bootstrap test (1000x replication) with outgroup and database retrieved from NCBI GenBank. From our perspective, this is the first report on finding fungal endophytes from rhizomes of Elettaria sp. yet further investigation is needed to evaluate their future bioprospectives.