Comparative transcriptomics reveals different strategies of Trichodermamycoparasitism

BMC Genomics

Research Article

Lea Atanasova¹  Verena Seidl-Seiboth¹  Sabine Gruber¹  Irina S Druzhinina²  Christian P Kubicek²  Fanny Coulpier³  Stephane Le Crom⁴ 

[1] Research Area Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstrasse 1a, A-1060, Vienna, Austria;Research Area Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstrasse 1a, A-1060, Vienna, Austria;Austrian Center of Industrial Biotechnology (ACIB), GmBH c/o Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstrasse 1a, A-1060, Vienna, Austria;École normale supérieure, Institut de Biologie de l’ENS, IBENS, F-75005, Paris, France;Inserm, U1024, F-75005, Paris, France;CNRS, UMR 8197, F-75005, Paris, France;École normale supérieure, Institut de Biologie de l’ENS, IBENS, F-75005, Paris, France;Inserm, U1024, F-75005, Paris, France;CNRS, UMR 8197, F-75005, Paris, France;UPMC Univ Paris 06, UMR7622, Laboratoire de Biologie du Développement, 9 quai St. Bernard, F-75005, Paris, France;CNRS, UMR7622, Laboratoire de Biologie du Développement, 9 quai St. Bernard, F-75005, Paris, France;

关键词: Hypocrea;    T. atroviride;    T. virens;    T. reesei;    Mycoparasitism;    Gene expression;    Biocontrol;    Transcriptomics;   

DOI  :  10.1186/1471-2164-14-121

 received in 2012-08-10, accepted in 2013-02-19,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundTrichoderma is a genus of mycotrophic filamentous fungi (teleomorph Hypocrea) which possess a bright variety of biotrophic and saprotrophic lifestyles. The ability to parasitize and/or kill other fungi (mycoparasitism) is used in plant protection against soil-borne fungal diseases (biological control, or biocontrol). To investigate mechanisms of mycoparasitism, we compared the transcriptional responses of cosmopolitan opportunistic species and powerful biocontrol agents Trichoderma atroviride and T. virens with tropical ecologically restricted species T. reesei during confrontations with a plant pathogenic fungus Rhizoctonia solani.ResultsThe three Trichoderma spp. exhibited a strikingly different transcriptomic response already before physical contact with alien hyphae. T. atroviride expressed an array of genes involved in production of secondary metabolites, GH16 ß-glucanases, various proteases and small secreted cysteine rich proteins. T. virens, on the other hand, expressed mainly the genes for biosynthesis of gliotoxin, respective precursors and also glutathione, which is necessary for gliotoxin biosynthesis. In contrast, T. reesei increased the expression of genes encoding cellulases and hemicellulases, and of the genes involved in solute transport. The majority of differentially regulated genes were orthologues present in all three species or both in T. atroviride and T. virens, indicating that the regulation of expression of these genes is different in the three Trichoderma spp. The genes expressed in all three fungi exhibited a nonrandom genomic distribution, indicating a possibility for their regulation via chromatin modification.ConclusionThis genome-wide expression study demonstrates that the initial Trichoderma mycotrophy has differentiated into several alternative ecological strategies ranging from parasitism to predation and saprotrophy. It provides first insights into the mechanisms of interactions between Trichoderma and other fungi that may be exploited for further development of biofungicides.

【 授权许可】

Unknown   
© Atanasova et al; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.