16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting

Brazilian Journal of Microbiology

Yendi Navarro-noya¹  César Hernández-rodríguez¹  Juan C Zenteno²  Beatriz Buentello-volante¹  Mario E Cancino-díaz¹  Janet Jan-roblero¹  Juan C Cancino-díaz¹ 

[1] ,UNAM Facultad de Medicina Departamento de BioquímicaMexico D.F ,Mexico

关键词: endophthalmitis;    Staphylococcus epidermidis;    PCR;    DGGE;   

DOI  :  10.1590/S1517-83822012000100033

来源: SciELO

【 摘 要 】

Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.

【 授权许可】

CC BY-NC   
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