Detection of hemotropic agents in a livestock farm using PCR and DGGE

Revista de Salud Animal

Bolívar, Ana María¹  Rosales, Datty¹  Rojas, Agustina¹  García-Lugo, Pablo¹  Torres, Yzoleth¹ 

[1] Universidad de los Andes, Mérida, Venezuela

关键词: cattle;    buffalo;    hemotropic agents;    PCR;    DGGE;   

DOI  :  

学科分类：兽医学

来源: Centro Nacional de Sanidad Agropecuaria

【 摘 要 】

This paper reports the results of a diagnostic evaluations by PCR for hemotropic agents in buffalo and cattle (n=77), existing in the same farm. There was a positivity of 74.03% of hemoparasite infection for the total population studied, prevailing Anaplasma marginale on Trypanosoma theileri, Trypanosoma vivax and Babesia bigemina, respectively. Additionally, some DNA problems were tested by DGGE, revealing the presence of A. marginale and Mycoplasma wenyonii. The results obtained confirm a presumptive clinical situation and the validity of the molecular method.

【 授权许可】

Unknown