Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1

Brazilian Journal of Microbiology

Zhikui Hao¹  Yujie Cai²  Xiangru Liao¹  Xiaoli Zhang²  Zhiyou Fang²  Dabing Zhang² 

[1] ,Jiangnan University School of Biotechnology Wuxi,People's Republic of China

关键词: chitinase;    medium optimization;    response surface method;    inulin;    Chitinolyticbacter meiyuanensis SYBC-H1;   

DOI  :  10.1590/S1517-83822012000100019

来源: SciELO

【 摘 要 】

The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.

【 授权许可】

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