Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145

Brazilian Journal of Microbiology

Mônica Caramez Triches Damaso²  Carolina M.m. Carvalho Andrade¹  Nei Pereira Jr² 

[1] ,Universidade Federal do Rio de Janeiro Escola de Química Departamento de Engenharia BioquímicaRio de Janeiro RJ ,Brasil

关键词: Xylanases;    Thermomyces lanuginosus;    corncob;    submerged fermentation;    medium optimization;    Xilanases;    Thermomyces lanuginosus;    sabugo de milho;    fermentação submersa;    otimização de meio;   

DOI  :  10.1590/S1517-83822002000400011

来源: SciELO

【 摘 要 】

In recent years, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile. Thermomyces lanuginosus IOC-4145 was able to produce a very high level of cellulase-free xylanase in shaken cultures using corncob as substrate (500 U/mL). An optimization of the medium composition in submerged fermentation was carried out aiming at a low cost medium composition for enzyme production. Statistical experiment design was employed for this purpose, pointing out corncob as the most important parameter, which affects enzyme production. Additionally, the influence of several chemicals on xylanase activity was investigated in the crude extract. A slight stimulation of the enzyme (5-15%) was achieved with NaCl and urea, both at 3 and 5 mM of concentration. On the other hand, dithiothreitol and beta-mercaptoethanol at a molarity of 5mM have caused a strong stimulation of the enzyme (40-53%). The crude xylanase displayed appreciable thermostability, retaining almost 50% of activity during 24 hours of incubation at 50ºC; about 50% of activity was present at 60ºC even after 4 hours of incubation. The enzyme also exhibited good storage stability at -20ºC without any stabilizing agent.

【 授权许可】

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